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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: SAMHD1 silencing cooperates with radiotherapy to enhance anti-tumor immunity through IFI16-STING pathway in lung adenocarcinoma

Fig. 1

SAMHD1 silencing caused cytosolic ssDNA accumulation in LUAD cells. A, B The mRNA levels of DNA end resection factors: SAMHD1, MRE11, CtIP, RAD50, NBS1 in H1299and H1975 cells with radiation were detected with qPCR. C Overall survival analysis of LUAD was performed using KM plotter. D, E Immunofluorescence was performed to detect the accumulation of ssDNA in the cytoplasm of H1299 and H1975 cells. F, G The accumulation of ssDNA in cytoplasm was detected by flow cytometry in H1299 and H1975 cells. The cytosolic ssDNA was evaluated by mean fluorescence intensity (MFI). Scale bar: 50 μm. N = 3; *, P < 0.05, **, P < 0.01, ***, P < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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