Fig. 2
Ang-(1–7) reduces systemic inflammatory response, alleviates myocardial injury, and increases survival in SIC mice. A Schematic representation of the animal experiments. Mice were injected with Ang-(1–7) (or same saline volume), IP, daily for 3 days. 30 min after administering the compounds (or saline) for 3 days of the administration, the animals in the corresponding groups were again IP injected by LPS (10 mg/kg) or saline. Echocardiography images were captured 12 h later and mice were sacrificed for further experiments. B–F Serum Ang II, IL-6, cTnT, IL-1β, and TNF-α levels (n = 6). G M-mode echocardiography representative images. H Quantitative assessment of LVEF, LVEDV, LVESV, LVID; s, LVID; d, LVFS in every mouse group (n = 4). I Representative images of HE staining. J Histopathological scores of the heart (n = 6). K mRNA levels of BNP in the myocardial samples (n = 4). L Influence of Ang-(1–7) pretreatment on the 3rd day of survival of septic mice (n = 12). NS: no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001