Fig. 1

KPNA2 was upregulated in a HUVEC hypoxia model and murine hindlimb ischemia model. A The gastrocnemius muscle was taken from the non-ischemic hindlimb and ischemic hindlimb of model mice at 1 days, 3 days, 7 days, and 14 days, and changes in KPNA2, KPNA1, KPNA3, KPNA4, and KPNB1 levels were detected by Western blotting. n = 5. B Changes in KPNA2, KPNA1, KPNA3, KPNA4 and KPNB1 levels in HUVEC at 0 h, 6 h, 12 h, 24 h and 36 h of hypoxia were detected by Western blotting. Each experiment was repeated three times. C The gastrocnemius muscle was harvested from the ischemic hindlimb of model mice at 14 days for KPNA2 immunofluorescence staining (red), the endothelium was labelled with CD31 (green), while the nuclei (blue) were stained with DAPI. Scale bar: 200 μm. n = 5. NonHLI non-hindlimb ischemia, HLI hindlimb ischemia