Fig. 7

ALA prevented glycolysis by promoting mitophagy/autophagy in lung fibroblasts. A–G PFKFB3 and HK2 in cell lysate were measured after treatment with Parkin siRNA, Mdivi-1, 3MA, bafilomycin A1, Rapa, and MG132. H, I Co-localization of LC3 or LAMP1 and HK2 or PFKFB3, measured by immunofluorescence. Bar: 20 μm. Data are presented as mean ± SEM. *P < 0.05 versus control group; ^#P < 0.05 versus TGF-β1 group; ^△P < 0.05 versus TGFβ1 + ALA group; ^&P < 0.05 versus TGFβ1 + ALA + bafilomycin A1 (n = 3)