Fig. 1

ALA attenuates bleomycin-induced lung fibrosis and TGF-β1-induced lung fibroblast activation. A Representative images of lung sections stained with H&E and Masson’s trichrome. Bar: 100 μm. B Ashcroft scores for each group. C Immunohistochemical staining was performed to determine the localization and expression of α-SMA. Bar: 50 μm. D Protein levels of collagen I and α-SMA in lung tissues were analyzed by western blotting. E, F Protein levels of collagen I and α-SMA. G mRNA levels of Col1a1 and Acta2 in fibroblasts. Original magnification: 200×. Data are presented as mean ± SEM. *P < 0.05 versus vehicle group; ^#P < 0.05 versus BLM group or TGFβ1 group; ^△P < 0.05 versus the TGFβ1 + ALA group (n = 3)