A simple and effective method to purify and activate T cells for successful generation of chimeric antigen receptor T (CAR-T) cells from patients with high monocyte count

Table 2 Optimal choice or range of process parameters in T cell isolation process defined by DoE

Process parameter	Optimal choice or range
Isolation medium	DPBS
T-cell density during Dynabeads incubation	410⁶–1010⁶ CD3⁺ cells/mL
Dynabeads incubation duration	30–60 min

Process parameters studied through DoE and their defined values are shown in the table

ISSN: 1479-5876