Table 1 Groups, factors, and factor levels of DoE
Group | CD3 + cells in PBMC (%) | Duration of incubation (min) | CD3 + cell density for incubation (e6 cells/mL) | Incubation buffer |
|---|---|---|---|---|
#01 | < 30 | 30 | 4 | X-VIVO15 |
#02 | < 30 | 30 | 10 | DPBS |
#03 | < 30 | 60 | 4 | DPBS |
#04 | < 30 | 60 | 10 | X-VIVO15 |
#05 | 30–60 | 45 | 7 | DPBS |
#06 | 30–60 | 45 | 7 | X-VIVO15 |
#07 | > 60 | 30 | 4 | DPBS |
#08 | > 60 | 30 | 10 | X-VIVO15 |
#09 | > 60 | 60 | 4 | X-VIVO15 |
#10 | > 60 | 60 | 10 | DPBS |