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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: A simple and effective method to purify and activate T cells for successful generation of chimeric antigen receptor T (CAR-T) cells from patients with high monocyte count

Fig. 1

CAR-T cell characteristics when using CAR-T culturing medium as the T cell selection buffer. This figure showed the outcome of CAR-T cell manufacture when CAR-T culturing medium was used as the T cell bead-based selection buffer. a The percentage of CD3 positive cells in PBMC before positive selection with anti-CD3/28 Dynabeads (BF) and positively selected cells after 44–52-h activation (AF). b The percentage of CD3+ cell population that express surface CD25 marker (b) or CD69 (c) before and after activation. d Cell diameter measurement results throughout the CAR-T production process. RV, the day of retroviral transduction and just before cell selection (AF). Day 1–7 indicates days after retroviral transduction. e Fold expansion of CAR-T cells after retroviral transduction. f Percentage of CD3+ cells express CAR at days 4 and 7 after retroviral transduction. g The percentage of live cells that were positive for CD14 surface marker before selection and after activation

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Journal of Translational Medicine

ISSN: 1479-5876

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