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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Low-intensity pulsed ultrasound triggers a beneficial neuromodulation in dementia mice with chronic cerebral hypoperfusion via activation of hippocampal Fndc5/irisin signaling

Fig. 7

Irisin drives reactive astrocytes toward neuroprotective phenotype. A Primary hippocampal astrocytes at DIV 12 were injured by OGD for 6 h, treated by recombinant irisin with different concentration, and then harvested 2days later. S100A10 level were assessed by qPCR. B Primary hippocampal astrocytes at DIV 12 were injured by OGD for 6 h, treated with LIPUS or/and recombinant irisin with different concentration, then harvested 2days later. S100A10 level were assessed by qPCR. C Representative images of primary astrocytes (green) expressing integrin-αVβ5 (red) in immunohistochemistry. D The integrin-αV and integrin-β5 level were determined using qPCR between the astrocytes with OGD injure and the astrocytes with OGD injure as well as LIPUS treatment. E Primary hippocampal astrocytes at DIV 12 were replaced medium with the NCM (OGD + LIPUS), simultaneously processed with 6 h OGD as well as 2 day LIPUS or/and the anti-integrin-blocking antibodies. S100A10 mRNA level were assessed by qPCR. (n = 6 per group, 6 independent experiments from 6 different astrocytes preps. All data are expressed as mean ± SEM **P < 0.01, ***P < 0.001, ns means no statistical significance)

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