Fig. 4

Downregulation of FOS attenuates the promotion of PHF5A overexpression on GC cells. A FOS was highly expressed in tumor tissues compared with adjacent normal tissues in GC patients. (The magnification is 200 × and 400 ×). B Cell proliferation in MGC-803 was measured using Celigo cell counting assay. Results were normalized to viability on day 1 and represented as fold change. C The clone number of MGC-803 cells was estimated by colony formation assay. D Apoptosis ability of MGC-803 cells was analyzed using Annexin V-APC staining, followed by flow cytometry. E, F Migration of MGC-803 cells was determined by Wound healing assay and Transwell assay. G Effect of PHF5A knockdown on MGC-803 cells stem was evaluated by Sphere-forming assay. The presented results were representative of experiments repeated at least three times. Data was represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001