Fig. 2

Knockdown down of PHF5A acts as a tumor suppressor in GC cells. A The effect of reduced PHF5A expression on the proliferation of AGS and MGC-803 cells was estimated by Celigo cells counting assay. B Flow cytometry was used to estimate the effect of reduced PHF5A expression on the apoptosis ability of AGS and MGC-803 cells. C, D Transwell and wound-healing experiments were performed to evaluate the effect of reduced PHF5A expression on AGS and MGC-803 cell migration. E Effect of PHF5A knockdown on GC stem cells was evaluated by Sphere-forming assay. The presented results were representative of experiments repeated at least three times. Data was represented as mean ± SD. **P < 0.01, ***P < 0.001