Fig. 7
METTL3 programs NOG destabilization via poly(A) tail shortening. A The remaining NOG mRNA in differentiated and undifferentiated DPSCs treated with actinomycin D was quantified by qPCR. B Protein translation was blocked by cycloheximide, and the remaining expression level of NOG was detected by western blotting (n = 3). C 3′-RACE characterization of the polyadenylation site and 3′ UTR information of NOG. D Diagram of 3′-RACE analysis of NOG mRNA. E The poly(A) tail length of the NOG transcript in METTL3 knockdown DPSCs was assayed by poly(A) tail length measurement. F Schematic of the m6A modification machinery in DPSC mineralization. Significance was determined via ANOVA or Student’s t test; the data are represented as the mean ± SD (n ≥ 3). *p < 0.05. **p < 0.01. ***p < 0.001