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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Stage-specific requirement for METTL3-dependent m6A modification during dental pulp stem cell differentiation

Fig. 2

METTL3 is required for functional DPSC differentiation. A Two independent shRNA lentiviruses were used to inhibit METTL3 expression, as confirmed by qPCR. B Western blotting detected the protein expression of METTL3 in DPSCs after METTL3 shRNA treatment (n = 3). C qPCR analysis of the mRNA expression of ALP, RUNX2 and DSPP in differentiated DPSCs (n = 3). D The protein expression levels of METTL3, RUNX2 and DSPP in METTL3 knockdown DPSCs under odontogenic induction were evaluated by western blotting. E The mRNA expression level of METTL3 in DPSCs after adipogenic induction for 0, 3, 7, and 14 days. F The mRNA expression of PPAR and LPL after adipogenic induction as detected by qPCR (n = 3). G The odontogenic differentiation of shMETTL3-1 and shCTR-1 lentivirus-transduced DPSCs was evaluated by ALP and ARS staining (n = 5). H The differentiation capacity of shMETTL3-2- and shCTR-2-transduced DPSCs after induction. I Representative images of Oil Red O staining in METTL3 knockdown DPSCs. OM-DPSCs: DPSCs induced by odontogenic medium. Significance was determined via ANOVA or Student’s t test; the data are presented as the mean ± SD (n ≥ 3). *p < 0.05. **p < 0.01. ***p < 0.001

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