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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Stage-specific requirement for METTL3-dependent m6A modification during dental pulp stem cell differentiation

Fig. 1

The dynamic m6A epitranscriptomic landscape in DPSC differentiation. A DPSCs were induced with osteo/odontogenic medium for 0, 7, and 14 days, and then the total m6A level in the RNA polls was quantified by the colorimetric method. B The m6A methylation landscape in the genome-wide transcriptome was evaluated by m6A RIP-seq. Distribution of m6A peaks in the 3′ UTR, CDS region and 5′ UTR. C Pie chart analysis of the m6A peak fraction in transcript segments. D Specific and conserved motif sequences of high-confidence m6A peaks identified by the HOMER database. E Epitranscriptome profile of m6A-tagged mRNAs in DPSCs after odontogenic differentiation. F Gene Ontology enrichment analysis of differentially m6A methylated and expressed transcripts. G The concentrations of SAM, SAH and potential of m6A establishment were qualified by LC‒MS/MS. Metabolites with a VIP value > 1 were further subjected to statistical analysis (n = 6). H The mRNA expression of METTL3, METTL14, and WTAP during DPSC mineralization, as evaluated by qPCR (n = 3). I The protein expression of METTL3, METTL14, and WTAP in DPSCs was evaluated by western blotting. SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine. Significance was determined via ANOVA or Student’s t test. The quantitative data are presented as the mean ± SD *p < 0.05. **p < 0.01. ***p < 0.001

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