Fig. 4

Mitochondrial function of cardiomyocytes after sepsis. A The content of malic acid in heart tissue was detected 2 h after resuscitation, n = 6/group. B Changes in the ATP level (3 independent experiments). C, D Mitochondrial maximum respiratory rate assay in NRCMs (3 independent experiments). E The mitochondrial membrane potential of NRCMs was observed by confocal microscopy (bar, 30 µm, 3 independent experiments). F Red/green fluorescent (Δψm) quantitative analysis in NRCMs. G The content of reactive oxygen species in NRCMs was observed by confocal microscopy (bar, 50 µm, 3 independent experiments). H ROS fluorescence quantitative analysis in NRCMs. I The mechanism of MR protecting mitochondria function of cardiomyocytes. a: P < 0.05 compared with the sham or normal group, b: P < 0.05 compared with the sepsis or LPS group, c: P < 0.05 compared with the LR group