Fig. 3

IPD enhances the antitumor activity of cytotoxic CD8⁺ T cells in breast cancer. A Representative contour plots and quantification of CD8⁺ T cells ratios in EO771 and 4T1 tumors (n = 5, t test). B, C Flow cytometry analysis of IFN-γ⁺ and TNF-α⁺ CD8⁺ T cells from EO771 tumor (B) and 4T1 tumor (C) (n = 5, t test). D Representative immunofluorescence images of CD8⁺ T cells (green) and granzyme B (red) in EO771 tumors. Scale bar, 25 μm. E, F Representative histogram of Ki-67 expression and percentages of Ki-67⁺ CD8⁺ cells in EO771 (E) and 4T1 (F) tumors (n = 5, t test). (G and H) Representative contour plots of PD-1⁺ Tim-3⁺ CD8⁺ T cells of EO771 (G) and 4T1 (H) tumors. Mean ± SEM; ** p < 0.01; *** p < 0.001; ns, not significant