Fig. 4

Calcitriol upregulates Mbnl1 expression and corrects aberrant splicing in skeletal muscle of HSA^LR mice.(a, c) Real-time RT-PCR analysis to estimate the expression of Mbnl1 in TA muscles (a) and soleus (c) of WT and HSA^LR mice treated with calcitriol as indicated. (b, d) Western blot analysis of MBNL1 in mouse TA muscles (b) and soleus (d). Left panel shows representative blots, and right panel shows the quantitative analysis of signal intensities. (e) RT-PCR analysis to evaluate the splicing of Clcn1 exon 7a, Serca1 exon 22 and Nfix exon 7 in TA muscles of four mice from each group as indicated. (f) The ratio of inclusion of relevant exon was calculated. The mean and SEM in each group are indicated. The data were analyzed by one-way ANOVA followed by Turkey multiple comparison correction. *p < 0.05, **p < 0.01 and ***p < 0.001