Fig. 1

Calcitriol upregulates MBNL1 expression in C2C12 myoblasts and myotubes. Real-time RT-PCR analysis (a, b) and western blot (c) were performed using undifferentiated or differentiated C2C12 cells. The cells were treated with calcitriol as indicated. Undifferentiated C2C12 cells were examined one day after the treatment. Differentiated C2C12 cells were examined on day 5 of differentiation. Expression levels of Mbnl1 mRNA and MBNL1 protein are normalized to those of Gapdh and GAPDH, respectively, and also to 0 nM-treated cells. Mean and SEM (n = 3 and 6 culture dishes for real-time RT-PCR and western blot, respectively) are indicated. *p < 0.05, **p < 0.01 and ***p < 0.001 by one-way ANOVA followed by Turkey multiple comparison correction