Fig. 7

SPTBN1 regulated the stability of GPT2 mRNA by binding to its 3’-UTR regions. A The half-life of GPT2 mRNA expression was measured by qRT-PCR after SPTBN1knockdown or overexpression in ccRCC cells. B Relative GPT2 mRNA level in ccRCC cells by RIP-PCR. C Dual-luciferase reporter assay showed the influence of SPTBN1 and GPT2 with wild-type or mutation 3’-UTR binding sites. D Cell growth of SPTBN1-knockdown ccRCC cells transfected with GPT2 siRNA or negative control siRNA. E Clonogenicity assay of SPTBN1-knockdown ccRCC cells transfected with GPT2 siRNA or negative control siRNA. F Lactate concentrations, glucose consumption rates and ATP concentrations of SPTBN1-knockdown 786-O cells transfected with GPT2 siRNA or negative control siRNA. G Lactate concentrations, glucose consumption rates and ATP concentrations of SPTBN1-knockdown Caki-1 cells transfected with GPT2 siRNA or negative control siRNA. (**: P < 0.05)