Fig. 5

SPTBN1 expression was negatively correlated with GPT2 expression. A Distribution of genes with a significant difference in expression between SPTBN1-knockdown and control 786-O cells. B qRT-PCR results about the relative GPT2 mRNA expression levels in SPTBN1-knockdown and control RCC cells. C qRT-PCR results about the relative GPT2 mRNA expression levels in SPTBN1-overexpression and control RCC cells. D Correlation analysis for the mRNA expression levels of SPTBN1 and GPT2 in the TCGA-KIRC cohort. E Correlation analysis for the mRNA expression levels of SPTBN1 and GPT2 in the NJMU ccRCC cohort 1 (N = 53) by qRT-PCR. F Correlation analysis for the protein expression levels of SPTBN1 and GPT2 form the NJMU ccRCC cohort 2 (N = 180) by IHC of TMA. G Representative IHC images of TMA for SPTBN1 and GPT2 expression in ccRCC patients. (**: P < 0.05)