Fig. 1

SPTBN1 was down-regulated in ccRCC. A The flowchart of our screening of Spectrin-family genes. B The heatmap of Spectrin-family genes expression patterns. C Boxplot showed the expression levels of Spectrin-family genes in TCGA-KIRC database. D The distribution of Spectrin-family genes from single-cell RNA-seq. E Dot plot of the expression of Spectrin-family genes in each cell. F Relative growth rate of RCC cell lines after transfection with small interfering RNA of SPTBN1 and SPTBN2. G The mRNA expression level of SPTBN1 in paired and unpaired samples from the TCGA-KIRC database. H The protein expression level of SPTBN1 in the CPTAC database and FUSCC-ccRCC Proteome cohort. I The receiver operating characteristic (ROC) curve of SPTBN1 among the TCGA-KIRC cohort. J qRT-PCR confirmed SPTBN1 was down-regulated in ccRCC tumor samples. K Western blot illustrated the expression of SPTBN1 in ccRCC clinical samples. L The expression of SPTBN1 in RCC cell lines by qRT-PCR and WB. (**: P < 0.05)