Fig. 1
From: A bispecific, crosslinking lectibody activates cytotoxic T cells and induces cancer cell death
Atomistic models for Stx1B K9AzK, OKT3 E129AzK and Stx1B-scFv OKT3 lectibody. a Side view of Stx1B containing AzK at the 9th position in each monomer. The structure is constructed based on PDB:1BOS. The five subunits of Stx1B are shown as red, pink, plum, purple and maroon ribbon cartoons; the AzK are given as sticks with grey color for C atoms, red for O atoms and blue for N atoms, H atoms are not shown for clarity. b Structural model of OKT3 E129AzK scFv (AzK is shown in sticks) built using homology modeling based on the template with OKT3 Fab from Mus musculus (PDB: 1SY6), the C-terminal fragment with low identity to the template sequence, including 6-His-tag appears as an unstructured tail. c Top view and d Side view of the Stx1B-scFv OKT3 lectibody model. Each Stx1B monomer is linked to one scFv OKT3 via DBCO-methyltetrazine-DBCO linker connected to the AzK residues. The resulting five subunits of the lectibody are shown in different colors