Fig. 4

GCN5L1-induced TFAM K76 acetylation inhibits the binding of TFAM to translocase TOM70. A Confocal microscopy for the location of acetylated TFAM and mitochondria in TECs. B Immunoblotting of TFAM interacting with TOM70, HSP70, TOM70, TOM40, TOM20, TIMM44 and TIMM17A from GCN5L1 overexpression TECs was identified by Co-IP. C Immunoblotting of TFAM interacting with TOM70, HSP70, TOM70, TOM40, TOM20, TIMM44 and TIMM17A from GCN5L1 knockdown TECs was identified by Co-IP. D The binding of TFAM with mitochondrial membrane translocases and chaperone protein including TOM70, HSP70, TOM40, TOM20, TIMM44 and TIMM17A was shown by Duolink proximity ligation assay in GCN5L1 overexpression TECs. Scale bars = 50 μm. E The binding of TFAM with mitochondrial membrane translocases and chaperone protein including TOM70, HSP70, TOM40, TOM20, TIMM44 and TIMM17A was shown by Duolink proximity ligation assay in GCN5L1 knockdown TECs. Scale bars = 50 μm