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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Estrogen antagonizes ASIC1a-induced chondrocyte mitochondrial stress in rheumatoid arthritis

Fig. 3

Estrogen decreased ASIC1a activity in articular chondrocytes. A The expression of ASIC1a decreased in a time-dependent manner following stimulation with 1000 nmol/mL estradiol for 0–48 h in chondrocytes. B Immunofluorescence analysis of ASIC1a expression in chondrocytes treated with PcTx-1, E2, and EGTA. The scale bars are 20 μm. C Calcium imaging detected the effect of ASIC1a on Ca2+ in chondrocytes treated with E2, PcTx-1. The scale bars are 20 μm. D Calcium imaging revealed ASIC1a-mediated Ca2+ influx with E2, PcTx-1. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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