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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Disruption of C/EBPβ-Clec7a axis exacerbates neuroinflammatory injury via NLRP3 inflammasome-mediated pyroptosis in experimental neuropathic pain

Fig. 1

Increased expression of Clec7a in spinal cord of the chronic constriction injury rats. (A) Mechanical withdrawal threshold in the ipsilateral paw of Sham and CCI rats (n = 12 rats/group). (B-C) Hierarchical clustering of differentially expressed genes (DEGs) in spinal cord (SC) between CCI and Sham rats using microarray analyses (B; n = 3 rats/group) and RNA sequencing (C; n = 4 rats/group). (D-E) Expression levels of Clec7a mRNA and protein in SC tissues of Sham and CCI rats determined by qPCR and western blot, respectively (n = 3 rats/group with 2 technical replicates). (F) Clec7a immunofluorescence in the ipsilateral spinal dorsal horns (magnification 5 × /20 ×) on Day 10 (post-nerve injury). White arrows point to Clec7a-positive cells. The mean fluorescence intensity (MFI) was calculated by Image J software (n = 4–6 rats/group, 1 section/rat, 1–2 fields of view/section). Scale bar: 200 μm (up panel), 50 μm (down panel). (G) Representative images and quantitative analysis of co-localization of Iba-1, GFAP or NeuN with Clec7a in the ipsilateral spinal dorsal horns between Sham and CCI rats (n = 3 rats/group, 1 section/rat, 3 fields of view/section). White arrows point to Clec7a-positive cells. Scale bar: 20 μm. Data are presented as mean ± SEM. Unpaired Student’s t test was used to analyze data between the two groups. *P < 0.05, **P < 0.01, ***P < 0.001 compared with Sham group.

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