Fig. 5

Yes-associated protein (YAP) plays an important role in mechanotransduction. A Relative mRNA expression levels of YAP and YAP target genes, ANKRD and CTGF, in HCCLM3 and Huh7 cells on hydrogel with different stiffness. B Representative immunofluorescence images of YAP (red) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) in HCCLM3 and Huh7 cells on hydrogel with different stiffness. Scale bar = 20 μm. C Quantification of YAP:DAPI nuclear co-localization is represented by Pearson’s correlation coefficient