Fig. 3From: Heterogeneous matrix stiffness regulates the cancer stem-like cell phenotype in hepatocellular carcinomaExpression of stem-like phenotypes of hepatocellular carcinoma (HCC) cells on polyacrylamide (PA) hydrogel with different stiffness. A Relative mRNA expression levels of NANOG and OCT4 in HCCLM3 and Huh7 cells on hydrogel with different stiffness. B Western blot (WB) for NANOG and OCT4 protein abundance in HCCLM3 and Huh7 cells on hydrogel with different stiffness. C Relative NANOG and OCT4 protein abundance was measured by quantifying band density using ImageJ software. After normalization to GAPDH protein expression for each sample, the semi-quantitate results were obtained as a ratio. D CD133-positive HCCLM3 cells cultured on hydrogel with different stiffness were estimated by flow cytometryBack to article page