Fig. 8

Immunohistochemical staining for AKR1B1, ITGAV, TGFβ1 and G6PD in matching ovarian tumor samples at diagnosis and at relapse. Immunohistochemistry on these samples was performed as described in Methods. Quantification of DAB staining for AKR1B1, ITGAV, TGFβ1 and G6PD was performed using Aperio ImageScope software. Mean ± SD; paired student’s t test; outliers greater than 2 SD are excluded where n = 3 in ‘at diagnosis’ group; *p < 0.05 in relapsed group when compared to initial diagnosis