Fig. 6

Cellular bioenergetics in OVCAR5 parental and OVCAR5 CBPR cells. Representative graph of measurements of (A) extracellular acidification rate (ECAR) following the sequential addition of glucose, oligomycin, an ATPase inhibitor and 2-deoxyglucose (2-DG), a competitive inhibitor for glucose of hexokinase, were used to calculate rate of B oxygen consumption rate (OCR), C oxidative phosphorylation (OXPHOS), D glycolysis, E non-glycolytic acidification and F glycolytic capacity. ECAR was normalized to total protein concentration. n = 3; mean ± SD; student’s t test; ****p < 0.0001, ***p < 0.001, *p < 0.05 when compared to OVCAR5 parental cells