Fig. 5

The effect of PSAT1 knockdown on osteogenic differentiation of PDLSCs in vitro and bone regeneration in vivo. A ALP staining of PDLSCs after osteogenic induction for 7 and 14 days. B Alizarin red staining of PDLSCs after osteogenic induction for 21 days. C Quantitative analysis of mineralized matrix after osteogenic induction for 21 days. D The quantitative analysis of ALP activity in PDLSCs after osteogenic induction. E The mRNA levels of COL1A1, ALP and RUNX2 in PDLSCs after osteogenic induction. F The protein levels of COL1A1, ALP and RUNX2 in PDLSCs after osteogenic induction. G, H Micro-CT assay of rat mandibular defect areas was performed after PDLSCs were transplanted, and the bone volume per tissue volume (BV/TV, %), the trabecular bone thickness (Tb. Th, mm), the trabecular bone separation (Tb.Sp, mm), and the total number of trabeculae (Tb.N; mm) were calculated. I HE staining and Masson’s trichrome staining of rat mandibular defect areas. White dotted line: bone defect boundary, n: new bone, f: collagen fiber, m: jaw bone. shPSAT1: PDLSCs with PSAT1 knocked down. shNC: control PDLSCs. *p < 0.05; **p < 0.01