Fig. 3

The effect of PSAT1 knockdown on proliferation activity and migration ability of PDLSCs. A PDLSCs transfected with lentivirus were observed under fluorescence microscope. B, C The expression levels of PSAT1 protein and PSAT1 mRNA in PDLSCs were detected by Western Blot and qRT-PCR. D Cell viability of PDLSCs was detected by CCK-8. E The distribution of the cell cycle in PDLSCs was detected by flow cytometry. F, H Cell proliferation activity of PDLSCs was detected by EdU, and the proportion of EdU-positive cells was calculated. G, I Migration ability of PDLSCs was analyzed, and the migration efficiency was calculated. shPSAT1: PDLSCs with PSAT1 knocked down. shNC: control PDLSCs. *p < 0.05; **p < 0.01