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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Discovery of a pyrano[2,3-b]pyridine derivative YX-2102 as a cannabinoid receptor 2 agonist for alleviating lung fibrosis

Fig. 5

YX-2102 suppressed TGFβ1-Smad2/3 signaling and decreased EMT-associated transcription factors. A549 cells were pretreated with YX-2102 (5.0 µM) for 2 h and stimulated with TGF-β1 (5 ng/mL) for 24 h. Control cells were treated with DMSO. A WB analyses of p-Smad2/3 expression. B Confocal analysis of A549 cells stained with pSmad3 (green) and Smad4 (red) antibodies after TGF-β1 stimulated with/out YX-2102. Nuclei were counter-stained with DAPI (blue). Scale bar = 10 μm. C IHC evaluating the expression of p-Smad3 in the lungs of rats at 21 days (magnification ×200). Scale bar = 200 μm. D A549 cells (untreated or treated with 5.0 µM YX-2102 for 24 h), were stimulated or unstimulated with TGF-β1 (5 ng/mL). mRNA levels of slug, snail, ZEB-1, and twist were then determined using RT-qPCR. Data are expressed as mean ± SEM of three independent experiments. **P < 0.01 versus Sham groups. ##P < 0.01 versus the TGF-β group

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