Fig. 3

YX-2102 inhibits BLM-induced pulmonary inflammation in vivo. Rats were intratracheally treated with 5 mg/kg BLM once at day 0, followed by YX-2102 or vehicle treatment via intraperitoneal injection at 25 mg/kg daily. The rats were sacrificed at day 7 and sampled. A Immunohistochemical staining of lung tissues using anti-MPO or CD68 antibody. B Representative images of cells in BALF after Wright-Giemsa staining. C The cell count of BALF using a hemocytometer and D the number of macrophage (Mφ), lymphocytes (Lym) and neutrophils (Neu). E, F The mRNA expression of IL-6, MCP-1, IL-1β (E) and IL-10, IL-4 (F). G, H The mRNA level of TNF-α, iNOS, CD86 (G) and Ym-1, Arg-1, CD206 (). I, J Double immunofluorescence staining of CD68 (red)/iNOS (green) (I) and CD68 (red)/CD206 (green) in rat lungs. Nuclei were stained with DAPI (blue). The merged signal (yellow) indicated M1 or M2 macrophages, respectively. Data were expressed as mean ± SEM, n = 5, ^**P < 0.01, ^***P < 0.001 versus Sham groups. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 versus BLM group