Fig. 7

Paxlovid accelerates cartilage degeneration and senescence in vivo. A–C Fast green/ Safranine O staining of the knee joint, and quantitative analysis of the OARSI score and proportion of Safranine O positive cartilage area. The black triangle depicts superficial abrasion of cartilage. The OARSI score was calculated as the sum of separate OARSI scores of cartilages in the femoral and tibial parts. Scale bar: 100 μm. D–J Immunofluorescence of Col2a1, p21, Ddit3, and Sod1 in articular cartilage, and related quantitative analysis of the proportion of immunofluorescence-positive cells. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001