Fig. 2

Schematic workflow of genome-wide CRISPR/Cas9 screening. A human genome-wide CRISPR/Cas9 knock-out library with sgRNAs is packed into lentiviral particles and transduced into Cas9-overexpressing cancer cells. The sgRNA-transduced cells are selected to generate mutant cells. Mutant cells are treated with drugs and DMSO (vehicle). DNA is extracted, and sgRNA is amplified via PCR. Whole-genome screening is conducted via next-generation sequencing before bioinformatics analysis. Volcano plots depicting genes selected with and without drug treatment and the corresponding networks are shown, with enriched genes on nodes and signaling pathways highlighted