Fig. 5
From: Chagasic cardiomyopathy is marked by a unique signature of activated CD4+ T cells
Regulatory T cells expressing CD39 are decreased in mild cardiomyopathy. Representative contour plots (A) and frequencies (B) of TREG, defined by the expression of FoxP3 and CD25, from controls and infected patients in different stages of Chagas disease (A, B1, B2-C-D). C Expression of CD39 and FoxP3 in cells from cluster MC6 (FlowSOM clusters 45, 49 and 50; top row) and combined FlowSOM clusters 46–48 (bottom row) between controls and patients are shown. Clusters are shown in blue and overlaid on total CD4+ T cells (grey). D The frequency of MC6 (top) and combined FlowSOM clusters 45, 49 and 50 (bottom) is shown for controls and patient groups. Bars represent the median and interquartile range. E MST shows the differential expression of FoxP3, CD25, and CD39, represented from blue to red indicating lower to higher expression, respectively. MC6 is highlighted by blue circles and FlowSOM populations 46, 47 and 48 by black circles. F Representative contour plots depict the expression of CD39 (blue) among TREG (gray). G Frequency of CD39+ T cells in CD4+ T cells (left), frequency of CD39+ in TREG (middle) and frequency CD39+TREG in CD4+ T cells (right). Bars represent the median and interquartile range. Asterisks represent significant differences between Chagas’ patients and controls. *p < 0.05, **p < 0.01, ***p < 0.001