Fig. 2
From: Chagasic cardiomyopathy is marked by a unique signature of activated CD4+ T cells
Unsupervised clustering of CD4+ T cells reveals unique characteristics of patients with Chagas disease. Minimum spanning trees (MST) show 100 clusters representing the numbers of each of the 50 FlowSOM populations (A) and their composition as defined by manual gating of CD4+ T cell subpopulations (TREG, TCM, TEM, TEFF, TN) (B). C Heatmap depicts the expression of 23 molecules, scaled by each marker, across the 50 FlowSOM populations. Proportion (middle panel) and composition (right panel) of each FlowSOM population within CD4+ T cells. FlowSOM populations were clustered based on hierarchical clustering by similar expression patterns and were further grouped in mesoclusters (MC1-7). These mesoclusters are highlighted in the MST (A). D Fold changes of FlowSOM population frequencies from infected patients in different stages of Chagas disease over controls. Colored bars highlight FlowSOM populations with the statistical difference between patients with clinical forms of Chagas disease (blue, A; orange, B1; coral, B2-C-D) and controls