Fig. 2
WZ35 can inhibit YAP from entering the nucleus. A HCCLM3 cell line was transfected with shRNA or pcDNA/peGFP vectors and harvested 48 h post-transfection to quantify the protein level of YAP and GAPDH. B and C Colony formation assay of different cell lines showed significant changes in stably transfected cells with representative images being shown and colony numbers being quantified. D CCK-8 assays were performed after cell lines were transfected with YAP shRNA or pcDNA/peGFP vectors. E The structure and synthesis of WZ35. F Western blotting analysis of the protein level of YAP, Cyr61 and CTGF in HCCLM3 cells treated with DMSO, curcumin and WZ35. G Western blot analysis of subcellular distribution of YAP in HCCLM3 cells treated with DMSO and WZ35.Histone H3 and Tublin are markers for nuclear and cytoplasm, respectively. Nuc., nucleus; Cyt., cytoplasm. H Representative immunofluorescent images via confocal microscopy were displayed with bars of 10 μm, confirming the reduction of nuclear localization of YAP protein. (Green: YAP; Blue: DAPI)