Fig. 5

Correlation analysis of HNRNPA2B1 and TLR4 expression in RPMI 8226 cells transfected with sh-HNRNPA2B1-8226 and control sh-NC-8226. A Differential motif between RPMI 8226 cells transfected with sh-HNRNPA2B1-8226 and sh-NC-8226. B IGV tracks indicated the m6A modification position of the TLR4 gene. C The percentage of peak distribution in different regions (5′UTR, CDS and 3′UTR) of sh-HNRNPA2B1-8226 and the control sh-NC-8226. D The distribution histogram of peak on four transcriptional functional regions (5′UTR, 3′UTR, CDs and intronic). E Heatmap of differential peak between MM cells transfected with sh-HNRNPA2B1-8226 and sh-NC-8226. F KEGG pathway enrichment results for differentially expressed genes identified by MeRIP sequencing between cells transfected with sh-HNRNPA2B1-8226 and control sh-NC-8226 in MM. TLR4 was enriched in many pathways of KEGG terms. G TLR4 gene expression in HNRNPA2B1 knockdown and control cells as analyzed by qRT-PCR. H TLR4 protein expression in HNRNPA2B1 knockdown and control cells as analyzed by western blotting