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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy

Fig. 3

HRS effects in neuronal apoptosis in the cerebral cortex and hippocampus in all groups 48 h after LPS administration (n = 5). A TUNEL immunoreactive stained sections showed increased neuronal apoptotic cell death in the LPS group compared to sham group, which was significantly decreased in the HRS-treated group. Scale bar = 50 µm. B, C Representation of mean number of TUNEL positive cells in the cerebral cortex and hippocampus. D Apoptotic cells were further stained with Hoechst 33258 showing dense blue appearance of apoptotic nuclei or fragmented cells. Increased bright blue stain apoptotic cells were observed in the LPS group compared to sham group, which were significantly decreased in the HRS-treated group. Data are presented as mean ± SD ****p < 0.0001, ***p < 0.001, **p < 0.005

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