Fig. 7
Study of the correlation between Ca2+ dysregulation and PKP2 expression. A Representative Ca2+ tracings in HC C-MSC treated with scramble shRNA or with shRNA selectively targeting PKP2 and loaded with Fura-2/AM. The measurements were performed on unstimulated cells cultured in GM. B–C The graphs represent respectively: B oscillation frequency (n = 93 out of 103 cells HC vs. n = 69 out of 92 cells Scramble vs. 170 out of 173 cells shRNA PKP2; One-Way Anova test) and C oscillation amplitude (n = 93 out of 103 cells HC vs. n = 69 out of 92 cells Scramble vs. 170 out of 173 cells shRNA PKP2; One-Way Anova test). D Representative images of WB analysis of proteins extracted from HC C-MSC treated with shRNA scramble or PKP2 cultured in GM, hybridized with anti-SERCA2 ATPase, anti-pCaMKII, anti-CaMKII, anti-IP3R, anti-STIM1, anti-CAV1.2 and anti-ORAI1 antibodies. Immunostaining of the housekeeping GAPDH or Tubulin are shown for normalization. For each protein, the densitometric analysis were reported as follows: SERCA2 ATPase (E), pCaMKII (F), CaMKII (G), IP3R (H), STIM1 (I), CAV1.2 (L) and ORAI1 (M) (n = 4 biological replicates) levels, normalized on GAPDH or Tubulin (Two-tailed Student’s t-tests). Data information: mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.0001