Skip to main content
Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Targeted delivery of ZNF416 siRNA-loaded liposomes attenuates experimental pulmonary fibrosis

Fig. 4

Loss- of function ZNF416 attenuates fibroblast differentiation, proliferation and contraction. A qRT-PCR analysis of relative expression of ZNF416 in MRC-5 cells treated with ZNF416 siRNA, with **p < 0.01 vs. control group. B Western blots and densitometric analysis demonstrated a dramatically decreased expression of fibrosis-related proteins after treatment of ZNF416 plasmid in MRC-5 cells, with **p < 0.01 vs. TGF-β1 + NC siRNA group. C Immunofluorescence analysis of Collagen I after treated with ZNF416 siRNA in MRC-5 cells, Collagen stained green, Nuclei were stained by DAPI, scale bar = 50 μm. D EdU assays indicated that ZNF416 siRNA attenuates the proliferation of MRC-5 cells, scale bar = 50 μm. E Effect of ZNF416 siRNA on the contractility of TGF-β1 induced fibroblast. F CCK8 assays were performed to evaluate cell proliferative ability in MRC-5 cells after ZNF416 knockdown, with **p < 0.01 vs. control group and #p < 0.05 vs. TGF-β1 + NC siRNA group

Back to article page