Fig. 1

ZNF416 expression is increased in mechanics-induced fibroblasts and fibrotic lung tissues. NIH/3T3 cells were cultured on 1 or 60 kappa collagen-coated hydrogels coverslips. A Protein levels of ZNF416 were determined by western blot. B mRNA levels of ZNF416 were determined by qRT-PCR, with ^**p < 0.01 vs. the 1 kappa group. C Immunofluorescence analysis and mean fluorescence intensity of ZNF416 in NIH/3T3 cells culture on 1 or 60 kappa collagen-coated hydrogels coverslips. ZNF416 stained green, Nuclei were stained by DAPI, scale bar = 25 μm, with ^**p < 0.01 vs. 1 kappa group. D Western blot analysis of ZNF416 and fibrotic markers including Fibronectin, Collagen I and α-SMA expression in mouse lungs of control (n = 3) and silicosis (n = 3) group. E Representative images of H&E staining and ZNF416 expression, and semiquantitative scoring of ZNF416-positive cells, as obtained from immunohistochemistry images in normal, silicosis or IPF lung tissues of human subjects by IHC, scale bar = 100 μm, with ^**p < 0.01 vs. the normal group