Fig. 1

Structure of the HiTIMED tumor-type-specific hierarchical model, library development, and cell projection. For each carcinoma type, 12 libraries in 6 hierarchical layers (Library L1 – Library L6B) were optimized to estimate cell proportions. The first layer uses a tumor-type-specific reference library to deconvolve the tumor cell fraction from other cell types (Library L1). The second layer uses a library to separate tumor, angiogenic, and immune components (Library L2). Similarly, the third to the six layers use libraries to deconvolve angiogenic and immune cell subtypes (Library L3A-L6B)