Fig. 6

The effect of HDACi on the secretion machinery of activated CD8⁺ T cells. A–E Age-dependent cytokines´ secretion pattern upon HDACi treatment. CD8⁺ T cells were activated with aCD3/aCD28 mAb and then treated or not with HDACi. After 2 days in culture the secreted amounts of several cytokines and enzymes were determined in the supernatant. Granzyme A and B are the only ones with a different scale than the others. Results are presented as ng/ml and pg/ml. N young = 5, N old = 6. *p value < 0.05, **p value < 0.01. F Relative transcript expression for selected enzymes and cytokines. SDHA and TBP were used as housekeeper genes. N = 5–7