Fig. 4

Relative protein and gene expression of selected genes. A Relative protein expression of selected proteins. The relative protein expression is presented as a ratio between the untreated and treated activated CD8⁺ T cells and it was normalized to the total protein staining with Ponceau S. For the modified proteins, an additional normalization was done to the unmodified protein. N = 5. B Representative blots for the relative protein expression. Two donors are presented for each condition and for each protein. C Relative transcript expression of genes involved in CD8⁺ T cell differentiation. The relative transcript expression of certain genes was calculated as described before and normalized to the same housekeepers. N = 5