Fig. 1

Effect of T-cell activation on the HDAC expression and acetylation profiles after HDACi treatment. A Relative gene expression of selected genes after 48 h of TCR-mediated activation of CD8⁺ T cells. Total RNA was isolated, and qPCRs were prepared for the selected HDACs, sirtuins and p300. SDHA and TBP are used as housekeepers. N = 10, *p value < 0.05. B Relative protein expression of selected HDACs after 48 h of TCR-mediated activation of CD8⁺ T cells. The relative protein expression is calculated as a ratio between the untreated and the treated cells and it is relative to the Ponceau total protein staining. N young = 6, N old = 7. C Representative blots for the selected HDACs. Two donors are presented for each condition and for all selected HDACs. E Relative expression of the acetylation profiles of activated CD8⁺ T cells after HDACi treatment. The relative expression of acetylated lysine was normalized to the Ponceau total protein staining and calculated as a ratio between untreated and HDACi treated samples. N = 5. D Representative acetylation blots. Two donors are presented for each condition. Red arrows indicate the bands with the most noticeable increase in acetylation