Fig. 5

Integration events occurring in exon/intron regions affect alternative splicing transcripts. A The scatter plot shows the number of differential alternative splicing transcripts based on the analysis of control non-transduced vs all transduced CAR T-cell products. The median and 25th-75th percentiles are indicated in lentiviral and γ-retroviral products. Student’s t-Test was used to calculate p-value. p > = 0.05 represents no statistical significance. AS transcripts, alternative splicing transcripts. B All differential alternative splicing transcripts are summarized according to different alternative splicing type. SE, skipped exon; RI, retained intron; MXE, mutually exclusive exon; A5SS, alternative 5’ splice sites, A3SS, alternative 3’ splice sites. C–E The Sashimi plots show integration events occurring at alternative exon/intron region and impaired skipped exon (C), retained intron (D), and mutually exclusive exon (E) process based on RNA-seq analysis. The height of the peaks shows exon coverage, the number in the red (orange) lines show the number of splicing reads. The black scheme under the plot shows the position of the gene in genome. Integration events are showed with blue/red horizonal line under alternative exon or intron position