Fig. 7
From: Temporal regulation of notch activation improves arteriovenous fistula maturation
Strategies of Inducible Switching Notch Signaling On and Off in VSMCs in vivo. A Scheme of creating the transgenic mice with doxycycline-induced dnMAML expression in VSMCs. B dnMAML1 was induced in the presence of doxycycline and thus blocks Notch activation; halting doxycycline treatment for the triple transgenic mice VSMCdnMAML1 reactivates Notch. C VSMCs isolated from the VSMCdnMAML1 mice showed increased dnMAML1 expression and decreased Notch expression after Doxycycline treatment. D CKD and AVF were created in VSMCdnMAML1 mice. Doxycycline was administrated in week 2, and the representative images of H & E and α-SMA staining were shown. E The areas of lumen and neointima were calculated (n = 5; *p < 0.05; Mann–Whitney test was used for statistical analysis; black scales = 50 µm; red scales = 200 µm)