Fig. 6
From: Temporal regulation of notch activation improves arteriovenous fistula maturation
Temporal and VSMC-Specific KO of RBP-Jκ Improves AVF Maturation. A and B Time course of the neointima formation in AVFs. CKD and AVFs were created in mice. AVFs were collected at indicated time after surgery. α-SMA immunostaining was performed. The representative images were presented (n = 3). C–E. CKD model and AVFs were created in control mice (E); or created in VSMCRBP−Jκ KO mice that were treated with tamoxifen before surgery (C) or 2 weeks after the AVF surgery (D). The H & E and immunostaining of RBP-Jκ and α-SMA staining were determined (C–E). F and G The areas of the neointima and lumen, as well as the ratio of neointima to lumen in AVFs created in Panel C–E were measured and calculated. (n = 5, *p < 0.05 compared with WT; #p < 0.05 compared with KO RBP-Jκ before AVF group; one-way ANOVA was used for statistical analysis; black and white scale bars = 50 µm; red scale bars = 200 µm)